Oxytetracycline (OTC) belonged to the tetracycline class of antibiotics, which entered the environment due to the use of human production and life. Oxytetracycline in the soil environment may affect its migration and transformation in the soil through different forces interacting with the soil, affecting its toxicity to organisms, and then causing serious harm to the soil ecological environment. Therefore, studied the environmental behavior of antibiotics in the soil can provide theoretical support for the treatment of soil environmental antibiotics. Using oxytetracycline as adsorbent and cold black soil as adsorbent, the adsorption behavior of oxytetracycline on different components of cold black soil was studied, and the effect of pH change on the adsorption of oxytetracycline in cold black soil was analyzed, and the adsorption mechanism of oxytetracycline was explored.The results show that under experimental conditions, the adsorption of different components of oxytetracycline in cold black soil is more in line with the Langmuir model, and multilayer adsorption and monolayer adsorption coexist, with monolayer adsorption as the mainstay; Soils that remove organic matter have the strongest adsorption capacity for oxytetracycline; The pH value has the greatest influence on the remove organic soil, the main mechanism is electrostatic action and bridging effect.